Greig Laidlaw will captain the side, following his stint as skipper in two matches during the June Tests in South Africa.
Teenage lock forward Jonny Gray is the only uncapped player in Scotland's 23-man squad to face South Africa at Murrayfield on Sunday.
Should Gray take to the field he will become one of 47 sets of brothers to have been capped for Scotland with his elder brother, British and Irish Lion Richie, restored to the second row in one of six changes to the side that started last weekend's 42-17 victory over Japan.
The other changes see Duncan Taylor replaces injured Matt Scott in the midfield; Alasdair Dickinson and Moray Low will start at prop and Jim Hamilton join Gray in the engine room.
Omitted from the squad this week in addition to the injured Scott are Euan Murray - absent for reasons of his religious faith - Tim Swinson, Alastair Kellock, Kelly Brown, Pat MacArthur and Henry Pyrgos.
Scotland: 15 Sean Maitland, 14 Tommy Seymour, 13 Nick De Luca, 12 Duncan Taylor, 11 Sean Lamont, 10 Ruaridh Jackson, 9 Greig Laidlaw (captain), 8 David Denton, 7 John Barclay, 6 Alasdair Strokosch, 5 Jim Hamilton, 4 Richie Gray, 3 Moray Low, 2 Ross Ford, 1 Alasdair Dickinson. Replacements: 16 Scott Lawson, 17 Ryan Grant, 18 Geoff Cross, 19 Jonny Gray, 20 John Beattie, 21 Chris Cusiter, 22 Duncan Weir, 23 Max Evans.