Pukke handed defending champions UP-Tuks their first defeat of the 2014 FNB Varsity Cup on Monday to claim top spot on the standings.
Coach Robert du Preez's team were ruthless in wet conditions in Pretoria as they shut out the home side to claim a convincing 18-0 victory and leapfrog them on the log in the process.
UCT recorded their third away win of the season in Johannesburg, scoring five tries on their way to a 37-29 victory over UJ to move up to third position.
The Ikeys have lost both of their home games this season, to table-topping teams UP-Tuks and NWU-Pukke, but have won all of their games on the road, while the defeat leaves UJ down in seventh place and facing a tough road to make the semifinals.
The top four after five weeks of action is rounded off by UFS-Shimlas who ended deadlocked at 13-13 with three-time champions Maties in their clash in Stellenbosch.
The wind played a major role at the Danie Craven Stadium as the visitors scored all of their points in the first half and the Maties scored all of their points after halftime to eventually share the spoils.
In the early game on Monday night NMMU were forced to work hard for their 18-12 victory over FNB Wits in driving rain at the Wits campus in Johannesburg.
The Eastern Cape side scored on the stroke of fulltime to secure the victory after the game had hung in the balance in the final stages.
Wits, for the second consecutive week, came close to notching their first-ever victory in the competition, but it was not to be as they remain rooted to the bottom of the table while the Madibaz moved up to fifth spot and remain within a shout of a playoff spot this year.
Meanwhile, in the Varsity Shield defending champions CUT scored seven tries on their way to a comprehensive 47-8 victory over the TUT Vikings who finished the evening at the bottom of the standings.
In Durban Peter de Villiers's WC fought their way back to secure a tight 33-32 victory over John Mitchell's UKZN Impi, who earned two bonus points as consolation to remain in second position.
In the Varsity Young Guns competition, the FNB Wits Young Guns suffered their third heavy defeat as they went down 103-0 to the NWU-Pukke Young Guns at home, while the Steinhoff Junior Maties secured a tough 26-16 win against the NMMU Young Guns in Stellenbosch.
In Steinhoff Koshuisrugby Championship action the Steinhoff Cobras from UCT got their campaign off to a promising start with a 28-13 victory over Steinhoff Majuba in Johannesburg and Steinhoff Patria from NWU-Pukke edged UP-Tuks outfit Steinhoff Onderstepoort 13-11 in Pretoria.